Today we
recognize Rosalind Franklin as a significant woman in the history of physics
because of her pioneering work in the area of X-ray diffraction. She is
well-known for being the first person to produce a clear diffraction image of
DNA, but she is slightly less well-known for her imaging of the tobacco mosaic
virus and the micro-crystal structure of coal. Some might argue that her
contributions make her a woman in the history of biology, which I do not deny. However,
we learn how to do X-ray diffraction in solid state physics and her mentor
and supervisor W. Lawrence Bragg won his Nobel Prize in the field of Physics for this
technique. Her methods are the methods of physics although she applied them to
biological structures.
Regarding
DNA, Franklin made a significant discovery, that DNA takes two crystal forms,
one for the wet orientation and another for the dry. It is the wet DNA that
appears in the famous Photograph 51 on which James Watson and Francis Crick
based their molecular model of DNA (without her permission) and later won their
Nobel Prize. Having particular knowledge of Franklin’s unpublished work, the
famous pair Watson and Crick was able to take more credit than they deserved
for what appeared to be scientific intuition. In 1962, Watson, Crick, and
Maurice Wilkins (Franklin’s team member) received the Nobel Prize in Physiology or Medicine. Franklin
deserved the Nobel Prize for her work. Franklin certainly should have been the
third person on the Nobel Prize Award; however, she had passed away four years earlier.
Many of the women on our list of significant women in the history of physics worked in Modern Physics concepts that are difficult to grasp (for high school students). However, Rosalind Franklin represents a good example of a woman
physicist who you can easily discuss her achievements in her classroom. Her
work on diffraction is something that high school students can understand,
because diffraction of light is easy to demonstrate in your classroom and most
people can understand the idea that since X-rays have much smaller wavelengths, they
will make diffraction images of much smaller objects. I have produced a video
on this topic—see below:
Female
students in particular are interested in the applications and the usefulness of
the ideas we learn in physics. To image the genetic blueprint of life is
certainly a useful application of both X-rays and diffraction. When we
understand the structure of a molecule or a virus, we have taken the most
important step to understanding and curing diseases that affect human life.
What Was the Experiment Like?
Franklin and
her lab assistant collected a small quantity of DNA and set it in the path of an
X-ray beam. The beam was created by accelerating electrons through a high
voltage and having them slam into a copper plate. This produced a nearly “monochromatic”
beam of X-rays. After passing through
the DNA, the X-ray beam was split or diffracted into fringes upon a
photographic plate. The center of this plate was obstructed by a small piece of
lead to block out the large amount of X-rays that would pass right through the
DNA. This enabled the diffracted rays to show up with greater contrast.
X-ray diffraction photo of DNA: Photograph 51
Photo credit:
http://www.bbc.co.uk/schools/gcsebitesize/science/add_edexcel/cells/dnarev3.shtml
The best photograph she took was Photograph 51. Clearly visible in this photograph: the
blank spot in the middle (the lead shield) and three diffraction spots on each
leg of the X. These spots tell us how tightly wound the DNA helix is and the
missing spot indicates the thickness of the DNA helix. Just by looking at this
picture, Watson and Crick were able to inform their model of DNA with the
appropriate scale and pitch (twistiness). They would not have been able to
build such a model without this most important clue provided by Rosalind
Franklin.
Biographies about Rosalind Franklin
The impact of biographies about Rosalind Franklin is powerful; they affect how we remember her and the importance of her work. New York Times reporter Robert McG. Thomas, Jr., wrote about the influence of the first definitive biography of Frank, entitled Rosalind Franklin and DNA, written by Anne Sayre and first published in 1975. The article noted: “Until Mrs. Sayre's book was published by Norton in 1975,
Rosalind Franklin had been little more than a footnote to the history of the
discovery of the double helix, the form taken by deoxyribonucleic acid, the
stuff of heredity.” The biography also touched on the perceptions and struggles of women in science: “Mrs. Sayre's book has been widely cited in feminist
circles for helping to expose rampant sexism in science.”
Rosalind Franklin and DNA
By Anne Sayre
Rosalind Franklin: The Dark Lady of DNA
By Brenda Maddox
Links and Resources Consulted
